Fig. 3

Microscopic view of COC, which was considered grade 1 before denudation and MII oocyte was obtained following denudation (A). (B) was considered grade 2 before denudation and MI oocyte was obtained after denudation. Morphologically, there was no difference between the PCOS group (B), which had low PTX3 and VDR mRNA expression, and the control group (A), in which both transcripts were normal, in terms of transparency, the number of cumulus cell layers, mosaic fragmentation and partial or complete loss of cumulus cells. The reason why the COC in B (grade 2) is surrounded by darker and compact cumulus cells than in A (grade 1) is due to the difference in COC grades, not the expression pattern of the cumulus genes. Scale Bars: 100 μm