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Fig. 5 | Reproductive Biology and Endocrinology

Fig. 5

From: Expression of NELL2/NICOL-ROS1 lumicrine signaling-related molecules in the human male reproductive tract

Fig. 5

In vitro characterization of molecular functions of human NELL2 and NICOL. (A) A schematic representation of recombinant protein expression and affinity purification. (B) Coomassie brilliant blue (CBB) staining of recombinant NELL2, recombinant COMP, BSA (left) and recombinant NICOL (right) proteins. (C) Immunoblot detection of recombinant human NELL2 (left) and human NICOL (right) with anti-NELL2 and anti-NICOL antibodies, respectively. (D) The association and dissociation of recombinant human NICOL bound to recombinant human NELL2 analyzed by surface plasmon resonance analysis. (E) Human ROS1 pulldown with human NELL2, COMP, and BSA. (F) Human ROS1 pulldown with human NICOL and BSA

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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