Fig. 3

Comprehensive transcriptome analysis of mRNA and enrichment analysis of biological function. (A) PCA illustrating the individual differences in the RNA-seq expression profiles between the PICK1 KO group and the control group. (B) Volcano plot highlighting differentially expressed mRNAs in oligoasthenospermia patients. The orange dots illustrate the upregulated DEGs, while the green dots illustrate the downregulated DEGs (adjusted P < 0.005 and log2 FCs > 0.5). (C) Heatmap of the top 25 DEGs. ‘Orange’ indicates high relative expression, and ‘green’ indicates low relative expression. (D) The top 10 most statistically significant pathways of enrichment analysis of differentially expressed genes. (E) The bubble diagram shows that more than half of these pathways are associated with vesicle trafficking