Table 3 Studies on differential expression of tsRNAs in infertility
From: Small RNAs, spermatogenesis, and male infertility: a decade of retrospect
Study | Population type/size | Sample | Methodology | Inference |
|---|---|---|---|---|
Hua et al. [29] | 87 normozoospermic infertile males undergoing IVF treatment | Sperm samples | Deep sequencing | Ten tsRNA [(Downregulated: GlyGCC-30-1, GlyGCC-30-2, ThrTGT-38, ThrTGT-39, and GluTTC-23). (Upregulated: ProAGG-32, ProTGG-32, ProAGG-31, AsnATT-20, ArgCCG-33)] were differentially expressed in High- and low-grade quality embryo. |
Han et al. [157] | 41 non-obstructive azoospermic, 15 obstructive azoospermic, 5 idiopathic oligospermic and 12 fertile controls | Seminal plasma extracellular vesicle | Sequencing and qRT-PCR | tRF-Val-AAC-010 and tRF-Pro-AGG-003 were found to be upregulated in non-obstructive azoospermic and oligozoospermic compared to healthy male. These can be the biomarker for diagnosis of NOA and idiopathic oligospermia patient. |
Chen et al. [159] | Human/ pooled five each (low, average and good quality embryo) | Embryos | Single cell RNA-seq | tsRNA-GLn-TTGs are differentially expressed in high- and low-quality sperms and also regulate cleavage of 3PN embryo. |