Fig. 7

CeRNA network of key lncRNA and qRT- PCR validation. (A) The PPI interaction network of dif-mRNAs in ceRNA network (only significant interactions were showed). Interactions with a combine score ≥ 0.7 were considered statistically significant. Each edge links two interacting genes. The color depth of each node correlates positively with its degree of connectivity. (B) Pearson’s correlation analysis of 10 genes in PPI network. (C) CeRNA network of four hub dif-lncRNAs. (D) CeRNA network of key dif-lncRNA (ENST00000429019). (E) The key lncRNAs-mRNAs from PPI network and ceRNA network were showed in Venn diagram. (F) CeRNA network of key lncRNA-miRNA-mRNA in the sankey diagram. (G) The relative expression levels ENST00000429019 and key dif-mRNAs in key-ceRNA network were validated by real-time PCR on a sample set of 8 URSA patients and 8 controls. (H) The relative expression levels ENST00000429019 and key dif-mRNAs in key-ceRNA network were validated by real-time PCR when knocking down ENST00000429019 in HTR8/SVneo trophoblast cells. Statistical analysis was performed by the Mann-Whitney U test. All data are shown as the mean ± SEM. * P < 0.05