Fig. 5

Ovarian cell apoptosis and impaired gene expression in succinate-administrated mice. A-C Western blot analysis of ovarian global Ksuc level in succinate-administrated mice in vivo. Scale bar: 100 μm. D and E TUNEL and IHC data on Cleaved-caspase 3 assay show an increase of apoptosis in ovarian cells mainly GCs of succinate-administered ovaries as compared with the control group. Asterisks indicate TUNEL-positive cells. Scale bar: 100 μm. The qRT-PCR (F) and western blot (G) and (H) analyses for mouse ovarian cell apoptosis, proliferation and P21, an ovarian aging-related marker. Error bars: SEM; (n = 5 mice / group). * P < 0.05, ** P < 0.01, *** P < 0.001, according to two-tailed Student’s t-test