Fig. 4

Effects of in situ ovarian administration of succinate on mouse ovarian function. A Ovarian appearance after in situ ovarian injection with NaCl (Ctrl, 40 mM) and succinate (40 mM). B and C Ovary weight (mg) and ovary index (ovary weight / body weight, mg/g). D and E Effects of high Ksuc level on serum anti-Müllerian hormone (AMH) and estradiol (E2) concentrations in mice after ovarian administration in vivo. F Histopathological examination and follicle counts of ovaries. The following different types of ovarian follicles were observed: primordial follicle (yellow arrow), primary follicle (green arrow), secondary follicle (blue arrow), antral follicle (red arrow), atretic follicle (black arrow). Scale bar: 100 μm. G–L Summary of follicle represented as the mean of three ovarian sections per mouse from the ovaries of two groups. * P < 0.05, ** P < 0.01