Fig. 1

Dnajb7 is a conserved gene that is highly expressed in postmeiotic germ cells. A Phylogenetic trees of DNAJB7 homologous proteins in mammalian species. The numbers in the dendrogram are bootstrap values (%). B, C, D Expression pattern of Dnajb7 mRNA from published human single-cell RNA-seq data (B) and published RNA-seq data of various types of mouse tissues (C) and isolated spermatogenetic cells (D). E qRT–PCR showing the expression pattern of Dnajb7 mRNA in different tissues of 10-week-old mice, n = 3. (F) qRT-PCR analyses of Dnajb7 mRNA levels in developing testes at postnatal Day 7 (P7), P14, P21, P28, P35 and P56, n = 3. G qRT-PCR showing the expression pattern of Dnajb7 mRNA across isolated spermatogenetic cells and mature spermatozoa, n = 3. PS, pachytene spermatocytes; RS, round spermatids; ES, elongating spermatids. SZ, spermatozoa. H Western blot analyses of the expression pattern of DNAJB7 protein in different tissues of 10-week-old mice. I Western blot showing the expression levels of DNAJB7 protein in developing testes at P7, P14, P21, P28, P35 and P56. J Western blot analyses of the expression levels of DNAJB7 in spermatogenetic cells isolated at P60. K Western blot analyses of DNAJB7 in subcellular fractions of adult testes. Lamin B1 and GAPDH served as nuclear and cytoplasmic protein controls, respectively. L Expression of EGFP-DNAJB7 fusion protein in HEK293T cells. Scale bar, 10 μm