Fig. 4

Validation of the functional PAR2 expression in mEEC. Ca²⁺ microfluorimetry. A Representative traces of 2-furoyl-LIGRLO-NH₂ (5 µM), the PAR2 agonist, stimulation of mEEC. B Percentage of oscillating cells in the responding cell population to trypsin (2 µg/ml), 2-furoyl-LIGRLO-NH₂ (5 µM) and the simultaneous application of either trypsin or 2-furoyl-LIGRLO-NH₂ with the specific PAR2 inhibitor I-191 (100 nM). Data is shown as mean ± SEM. *** p < 0.001 compared to the trypsin application. Statistical significance was analyzed with a one-way ANOVA corrected for multiple comparisons with Dunnett’s multiple comparison test. C-D Trypsin or 2-furoyl-LIGRLO-NH₂ responses were challenged with the inhibitor of PAR2, I-191 (100 nM). Ionomycin was added at the end of each experiment as a positive control. N = at least 3 different experiments with a total minimum of 300 cells