Fig. 5

Hyperacetylation of α- tubulin in HtrA2-KD oocytes. (A) Metaphase II oocytes were labeled with an anti-acetylated α-tubulin antibody (green) and counterstained with propidium iodide to visualize the chromosomes (red). Representative images of control and HtrA2-KD oocytes are shown. Scale bar: 5 μm. (B) Quantification of fluorescence intensity from acetylated tubulin in control (n = 10) and HtrA2-KD (n = 34) oocytes. (C) Representative western blots showing the expression of acetylated tubulin in oocytes from control and HtrA2-KD mice (n = 100 per group). The relative amount of HtrA2 was estimated based on the level of actin and GAPDH. (D) Quantitative RT-PCR analysis of gene expression in control and HtrA2-KD oocytes. *, p < 0.05. HtrA2-KD, use siRNA-1