Fig. 7

TSC1 and DKK3 are direct targets of miR-92b-3p. A B The conservation of the miR-92b-3p binding sites in the 3’UTR of DKK3 and TSC1 from 7 different species, and the predicted binding site and mutated site of miR-92b-3p in the 3’UTR of porcine DKK3 or TSC1. C D PTr2 cells were co-transfected with PmirGLO-DKK3-3' UTR (Wt or Mut) or PmirGLO-TSC1-3' UTR (Wt or Mut) and the indicated RNA oligonucleotides (NC and miR-92b-3p). * P < 0.05, * *P < 0.01. RT-qPCR (E) (F) and Western blot (G) (H) (I) analysis of DKK3 and TSC1 after transfection with miR-92b-3p mimic or inhibitor in PTr2 cells. J EVs-miR-92b-3p inhibited the mRNA expression level of DKK3 and TSC1; K L After the treatment of EVs-miR-92b-3p, the relative protein expression level of TSC1 and DKK3. The β-actin was used as the internal control of Western Blot. Data are mean ± s.d., derived from three independent experiments. * P < 0.05, * *P < 0.01