MicroRNAs secreted by human preimplantation embryos and IVF outcome

Table 2 Quantitative RT-PCR on miRNAs in spent blastocyst culture medium

	miRNA	Group I	Group II	Group III	Group IV
Internal standard gene	cel-miR-39
ΔCt	hsa-let-7c-5p	12.0	12.0	11.0	11.9
	hsa-let-7i-5p	8.4	8.4	7.9	8.5
	hsa-miR-148a-3p	10.7	9.1	6.4	10.3
	hsa-miR-16-5p	9.5	9.9	5.7	16.4
	hsa-miR-181a-5p	7.5	8.8	8.9	10.6
	hsa-miR-191-5p	6.5	6.7	3.7	6.9
	hsa-miR-192-5p	3.7	3.8	2.9	3.6
	hsa-miR-30e-5p	−5.3	−6.0	−6.8	−6.1
	hsa-miR-320a	6.9	7.6	3.5	9.4
	hsa-miR-92a-3p	5.8	5.9	2.5	7.9
	hsa-miR-99b-5p	10.3	10.5	12.5	20.9
	hsa-miR-25-3p	8.4	8.4	4.5	9.9
	hsa-miR-509-3p	0.0	−0.2	−0.7	−0.6
	hsa-miR-378a-3p	9.6	9.5	4.7	10.0
	hsa-miR-372–3p	4.5	5.6	−1.3	NaN
	hsa-miR-28-3p	10.6	10.2	6.8	11.0
	hsa-miR-371a-5p	7.6	9.6	3.2	NaN
	hsa-miR-373-3p	5.2	6.4	−0.6	9.7
	hsa-miR-512-5p	5.9	5.9	2.1	6.5
	hsa-miR-320b	8.6	9.4	6.0	10.8

In addition to the 15 miRNAs that showed significantly different expression levels between the groups in the RNA sequencing analysis, five miRNAs suggested to contribute to embryonic maturation and implantation in literature were assessed. Their expression in the culture medium of individual blastocysts was analyzed using relative quantitative analysis by Taqman realtime PCR. We found that embryos with good morphology showed higher ΔCt values than those with poor morphology, and that the pregnancy group tended to show higher ΔCt values than the non-pregnancy group

ISSN: 1477-7827