Table 1 Sequences of transcript primers and annealing temperatures for RT-PCR
mRNA Target | Primer Sequence: 5’-3’ | Annealing temperature | Acession Numbera | Ref |
|---|---|---|---|---|
GADPH | Forward-GGATGATGTTCTGGAAGAGCC | 55ºC | - | [15] |
Reverse-AACAGCCTCAAGATCATCAGC | ||||
IL-1β | Forward-ATGATGGCTTATTACAGTGGCAA | 60ºC | - | [20] |
Reverse-GTCGGAGATTCGTAGCTGGA | ||||
TNF-α | Forward-GCTGCACTTTGGAGTGATCG | 60ºC |  |  |
Reverse-GTGTGCCAGACACCCTATCT | ||||
CCL18 | Forward-TGCATTGCAGCGTCATCTTG | 57ºC | NM_002988.4 | - |
Reverse-GAGTCCCATCTGCTATGCCC | ||||
CCL22 | Forward-AGGGCCAGGGGACATCTAAT | 57ºC | NM_002990.5 | - |
Reverse-GAGATCTGTGCCGATCCCAG | ||||
COX-2 | Forward-GTTCCACCCGCAGTACAGAA | 58ºC | NM_000963.4 | - |
Reverse-AGGGCTTCAGCATAAAGCGT | ||||
5-LOX | Forward-CATGCCCAGGAACAGCTCGTT | 57ºC | - | [21] |
Reverse-AGTCCTCAGGCTTCCCCAAGT | ||||
12-LOX | Forward-GCCCAAAGCTGTGCTAAACC | 58ºC | NM_000697.3 | - |
Reverse-TGGGGGAGGAAATAGAGCCT | ||||
15-LOX | Forward-CCTTCGTCCTCCAAACCTGT | 57ºC | NC_000017.11 | - |
Reverse-GCTCGGATGTGGGTAGTGAC |