Table 1 Comparison of 2D, 3D and 3D organoid culture models in studies
| Â | 2D model | 3D model | 3D organoid model |
|---|---|---|---|
Physiological recapitulation | Relatively low | Relatively high recapitulation but may be artificial | Highly recapitulate the tissue of origin |
Cell-ECM interaction | Lack of interaction | Mimic Natural ECM environment | Mimic Natural ECM environment |
Cell types | Usually only one cell type involved | Multiple cell types involved | Multiple cell types involved |
Stability of the culture system | Very stable for cell lines but relatively less stable for primary cells | Usually not stable for a long time | Stable culture system |
Ease of setup/cost | Easy to set up. Low cost | Set up requires high expertise. Relatively high cost | Specific culture medium required. Very high cost |