Fig. 4

IGF2 ameliorates the quality of obese mice oocytes by reducing oxidative stress. A Morphology of spindle and chromosome organization in ND, HFD, and HFD + IGF2 oocytes. α-tubulin antibody (green) was used to stained the spindles while chromosomes were counterstained with Hoechst 33342 (blue). Scale bar = 10 μm. B Quantification of oocytes with spindle/chromosomes defects in ND (n = 38), HFD (n = 76), and HFD + IGF2 (n = 72) oocytes. C Quantification of ROS (DCFH-DA staining) signals in ND (n = 10), HFD, (n = 10), and HFD + IGF2 (n = 10) oocytes. D Representative confocal images showing CM-H2DCFDA fluorescence (green) in ND, HFD, and HFD + IGF2 oocytes. Scale bar = 20 μm. E Adenosine triphosphate (ATP) content in ND (n = 50), HFD (n = 50), and HFD + IGF2 (n = 50) oocytes. *p < 0.05. One-way ANOVA test was used for statistical analysis. Error bars indicate the SEM