Table 2 Details of antibodies used for western blot
From: Diverse actions of sirtuin-1 on ovulatory genes and cell death pathways in human granulosa cells
Antibody target | Dilution | Species specificity | Source | Supplier | Catalog no. |
|---|---|---|---|---|---|
MCL1 | 1:1000 | Human | Rabbit | Cell signaling Technology (Danvers MA, USA) | CS-5453 |
BCL-XL | 1:1000 | Human | Rabbit | Cell signaling Technology | CS-2762 |
PARP | 1:1000 | Human | Rabbit | Cell signaling Technology | CS-9532 |
Full-length Caspase 3 | 1:1000 | Human | Rabbit | Cell signaling Technology | CS-14220 |
Cleaved Caspase 3 | 1:1000 | Human | Rabbit | Cell signaling Technology | CS-9664 |
RIPK1 | 1:1000 | Human | Rabbit | Cell signaling Technology | CS-14993 |
MLKL | 1:1000 | Human | Rabbit | Cell signaling Technology | CS-3493 |
Total p44/42 MAPK | 1:5000 | Human | Rabbit | Millipore (Burlington, MA, USA) | ABS44 |
HRP-linked IgG | 1:10,000 | Anti-rabbit | Donkey | Jackson immunoResearch (West Grove PA, USA) | 711–035-152 |