Fig. 3
UFL1 depletion aggravates cisplatin-induced ER stress and apoptosis in GCs, with lower MMP and increased mitochondrial dysfunction. A The knock down efficiency of UFL1 shRNA. B UFL1 knockdown attenuated GCs colony formation. (C) The proliferative activity of GCs was evaluated by the EdU staining positive cell rate. Bar, 100 μm. D Changes in the protein expression of UFL1, GRP78, XBP1s and CHOP. (E) IF was used to detect the expression of GRP78 between the control and UFL1 knockdown GCs. Bar, 50 μm. F Changes in UFL1, BAX, BCL-2, cleaved caspase-3 and caspase-3 protein levels. G The mitochondrial membrane potential was measured by TMRE staining and measured by flow cytometry. H E2 secretion from GCs was detected by ELISA. n ≥ 3 for each group. * p < 0.05; ** p < 0.01; *** p < 0.001 compared with the control group. # p < 0.05 ## p < 0.01; ### p < 0.001 compared with the cisplatin treatment group