Fig. 5

Effects of MHY1485 on chromosome alignment and DNA damage levels of IVM oocytes. A The immunofluorescence images of chromosome and the percentages of chromosome aberration in IVM oocytes from four groups, including control, 10 nM rapamycin-treated, 250 μM MHY1485-treated, and mix group (adding both rapamycin and MHY1485). Scale bar, 25 μm; B The fluorescence images of DNA and γ-H2AX in oocytes from the four groups. Scale bar, 20 μm. Con: control; Rapa: rapamycin; MHY: MHY1485. *P < 0.05; **P < 0.01; ***P < 0.001