Fig. 4

Effect of BMP type I receptor inhibitors on the BMP2-induced upregulation of CTGF expression in SVOG cells. A and B SVOG cells were pretreated with dorsomorphin (10 μM) for 1 h, and then the cells were treated with Ctrl or BMP2 (50 ng/ml) for an additional 6 h (A) or 12 h (B). The mRNA (A) and protein (B) levels of CTGF were examined using RT-qPCR and western blot analysis, respectively. C and D SVOG cells were pretreated with DMH-1 (0.25 μM) for 1 h, and then the cells were treated with Ctrl or BMP2 (50 ng/ml) for an additional 6 h (C) or 12 h (D). The mRNA (C) and protein (D) levels of CTGF were examined using RT-qPCR and western blot analysis, respectively. The results are expressed as the mean ± SEM of at least 3 independent experiments, and values without common letters are significantly different (P < 0.05)