Fig. 4

Inhibition of miR-218-5p prevents the epithelial-mesenchymal transition. A Immunofluorescent analysis of Vimentin (Red) and LASP1 (Green) in the control eutopic endometrium (CEu), adenomyosis eutopic endometrium(AEu) and adenomyosis ectopic endometrium(AEc) respectively. DAPI staining was included to visualize the cell nucleus (Blue), Scale bar = 100 μm. B and C Statistic analysis of Immunofluorescent staining of LASP1 and VEGF-α in the gland epithelial cell of three groups. D and E Statistic analysis of Immunofluorescent staining of LASP1 and VEGF-α in the stromal cell of three groups: CEu (n = 17), AEu (n = 16) and AEc (n = 16). P-values were determined by Student’s t-test, ***p < 0.001. F Western blot analysis showing effects of miR-218-5p on protein levels of Vimentin. GAPDH was used as the loading control