Fig. 1

Dose response measured by the XTT assay (OD at 450–650 nm). HTR-8/SVneo cells were treated with anthracene, benzo[k]fluoranthene, benzo[a]pyrene, and 4,4-(9-fluorenylidene)diphenol for the cell viability analysis using XTT assay. The absorbance prior to anthracene exposure was 1.530 ± 0.029, which decreased with an increase in anthracene concentration. In HTR-8/SVneo cells treated with increasing concentrations of benzo[k]fluoranthene, the absorbance decreased with an increase in the concentration. Treatment with 300 µg/mL benzo[k]fluoranthene resulted in an OD of 1.500 ± 0.011, which was lower than that of the control. Following treatment with benzo[a]pyrene at concentrations of 10 µg/mL and 100 µg/mL, the absorbance was lower than that of the control. Treatment with 4,4-(9-fluorenylidene)diphenol, which is a substitute for BPA, showed a decrease in absorbance at a concentration of 5 µg/mL