Fig. 7

MA promoted cisplatin-induced cytotoxicity in granulosa cells, and cisplatin-induced injure in the ovary. A granulosa cells were incubated with different concentrations of MA for 48 h, the cell viability was measured by cck-8 assay. B granulosa cells were incubated with selected cisplatin in the presence or absence of MA for 48 h, and the cell viability was measure by cck-8 assay. C-G The western blotting showed that MA increased the cisplatin-induced BAX and BNIP3 expression and decreased the cisplatin-induced FTO and Bcl-2 expression. One representative experiment of three independent experiments is shown. The results represent the mean ± SEM from three independent experiments. one-way ANOVA was used to compare the multiple groups. H HE staining suggested MA promoted cisplatin-induced injure in the ovary. (n = 15 mice per group). (*P < 0.05, **P < 0.01, ***P < 0.001, ns, no significance)