Fig. 4

The effects between circ_FURIN and miR-423-5p on TTR-mediated proliferation and apoptosis. A qRT-PCR was used to determine the impacts between circ_FURIN knockdown and miR-423-5p depletion on the miR-423-5p expression. B-G KGN cells were treated with TTR, TTR + sh-NC, TTR + sh-circ_FURIN, TTR + sh-circ_FURIN + anti-miR-NC or TTR + sh-circ_FURIN + anti-miR-423-5p, with untreated KGN cells as a control, and cell proliferation was analyzed by EdU, MTT and flow cytometry analysis for cell cycle assays (B-D), the protein expressions of PCNA, Bcl-2, Bax and Cleaved PARP by Western blot (E and G), and cell apoptosis by flow cytometry analysis for cell apoptosis (F). Statistical significances were compared by one-way analysis of variance with Tukey’s test. *P < 0.05