Fig. 2

The effects of circ_FURIN knockdown on TTR-induced proliferation repression and apoptosis promotion. A The efficiency of circ_FURIN knockdown was determined by qRT-PCR in KGN cells. B-G KGN cells were treated with TTR, TTR + sh-NC, and TTR + sh-circ_FURIN, with mock KGN cells as a control, and cell proliferation was analyzed by EdU, MTT and flow cytometry analysis for cell cycle assays (B, C and E), the protein expressions of PCNA, Bcl-2, Bax and Cleaved PARP by Western blot (D and G), and cell apoptosis by flow cytometry analysis for cell apoptosis (F). Statistical significances were compared by Student’s t-tests in (A) and one-way analysis of variance with Tukey’s test in (B-G).*P < 0.05