Fig. 3

Expression and production regulation of SAA1 in human granulosa cells. A Immunofluorescence staining of SAA1 (green) in cultured granulosa cells. Nucleus was stained with DAPI (blue). Scale bars, 50 μm. B, C Effects of IL-1β (1 ng/ml for 24 h) on SAA1 mRNA expression (B) and secretion (C) (n = 4). * P < 0.05, ** P < 0.01 compared with control (0). D Concentration dependent effect of SAA1 (0, 0.01, 0.1 and 1 μg/ml for 24 h) on SAA1 expression in cultured granulosa cells (n = 4). * P < 0.05 compared with control (0), # P < 0.05 compared with SAA1 (0.01 μg/ml). E Effects of OxpAPC (30 μg/ml), a TLR2/4 inhibitor and JSH-23 (10 μM), a p65 inhibitor on the induction of SAA1 expression by SAA1 (1 μg/ml) per se (n = 3). * P < 0.05 compared with control (0), # P < 0.05 compared with SAA1. All values are the mean ± SEM