Fig. 6
From: Pak2 reduction induces a failure of early embryonic development in mice
Pak2-KD induces apoptosis of early mouse embryos. (A) TUNEL analysis of control and Pak2-KD embryos. Embryos were labeled with Hoechst 33342 (blue) for DNA and TUNEL used to assess fragmented DNA (red). Arrows point to apoptotic cells in blastocysts (scale bars, 20 μm). (B) Quantification of control and Pak2-KD group blastocysts with TUNEL- positive nuclei (8.9 ± 0.32, n = 50 vs. 32.8 ± 1.25, n = 37; respectively). Data are expressed as mean percentage ± SD from three independent experiments. *Significantly different (p < 0.05). Pak2, p21-activated kinase 2; SD, standard deviation; Pak2-KD, Pak2 knockdown; TUNEL, terminal deoxynucleotidyl transferase dUTP nick-end labeling