Fig. 5
From: Pak2 reduction induces a failure of early embryonic development in mice
Pak2 knockdown results in the accumulation of γH2AX during preimplantation embryonic development. (A) Two-cell embryos were immunostained with an anti-γH2AX antibody to detect DNA damage (green) and counterstained with PI for DNA (red). Arrows indicate the increased DNA damage in embryos (scale bars, 20 μm). (B) Quantification of the numbers of γH2AX foci in control and Pak2-KD group embryos (94.3 ± 2.59, n = 32 vs. 234.0 ± 4.31, n = 37; respectively). Data are expressed as the mean ± SD from three independent experiments. *Significantly different (p < 0.05). Pak2, p21-activated kinase 2; Pak2-KD, Pak2 knockdown; SD, standard deviation; PI, propidium iodide