Icariin improves testicular dysfunction via enhancing proliferation and inhibiting mitochondria-dependent apoptosis pathway in high-fat diet and streptozotocin-induced diabetic rats

Table 1 Primer pairs and corresponding annealing temperatures used in this study

Gene	Sequence(5′-3′)	Annealing Temperature	Product (bp)
PCNA	F: GCTCCATCCTGAAGAAGGT R: TGCACTAAGGAGACGTGAGA	55 °C	121
Bax	F:GAGACACCTGAGCTGACCTT	55 °C	104
Bax	R:TCCATGTTGTTGTCCAGTTC	55 °C	104
Bcl-2	F:AGTACCTGAACCGGCATCT	55 °C	120
Bcl-2	R:TCTTCAGAGACAGCCAGGA	55 °C	120
Caspase-3	F:CCGGTTACTATTCCTGGAGA	55 °C	117
Caspase-3	R:TAACACGAGTGAGGATGTGC	55 °C	117
SIRT1	F:GTGGCAGTAACAGTGACAGTG R:GTCAGCTCCAGATCCTCCAG	55 °C	143
HIF1α	F:GATGGAATGGAGCAGAAGAC R: CACAATCGTAACTGGTCAGC	55 °C	112
CAT	F: ACAACTCCCAGAAGCCTAAGAATG R: RGCTTTTCCCTTGGCAGCTATG	55 °C	76
Gpx	F: GGAGAATGGCAAGAATGAAGA R: RCCGCAGGAAGGTAAAGAG	55 °C	139
SOD	F: CGAGCATGGGTTCCATGTC R: CTGGACCGCCATGTTTCTTAG	55 °C	101
GAPDH	F: CCTCAAGATTGTCAGCAATG R: CAGTCTTCTGAGTGGCAGTG	55 °C	134

ISSN: 1477-7827