Fig. 5

WEE1 promotes activation of the Wnt/β-catenin signaling pathway in ESCs. A Western blot analysis of β-catenin protein levels in WEE1 overexpressing or knockdown ESCs. Relative β-catenin protein levels are presented as mean ± SD. B Western blot analysis of WEE1, β-catenin, α-SMA and Collagen I protein levels in WEE1-overexpressing ESCs treated with the selective Wnt/β-catenin inhibitor, XAV939. Relative protein levels are presented as mean ± SD. C Immunofluorescence was used to detect the expression of α-SMA and Collagen I in ESCs. Scale bar: 50 μm. * p < 0.05; ** p < 0.01; *** p < 0.001 compared to control cells