Fig. 4

Inhibition of WEE1 prevents endometrial fibrosis in mice. A Representative abdominal findings from control mouse 3 weeks after induction of endometriosis. Endometriotic lesions are mainly observed as small cysts. B Protein levels of WEE1 in eutopic and ectopic endometrial tissues from a mouse model of endometriosis compared with normal mouse endometrial tissues by western blotting. C Expression of WEE1 in eutopic and ectopic endometrial tissues from a mouse model of endometriosis and in endometrial tissues from control mice was assessed by immunohistochemistry. D Western blot analysis of WEE1, α-SMA and Collagen I protein expression in the uterine tissue from control, Estradiol-, AZD1775- or Estradiol+AZD1775-treated mice 3 weeks after induction of endometriosis. E Representative immunohistochemistry images showing α-SMA, Collagen I and Masson’s trichrome staining in the uterine tissue of control, Estradiol-, AZD1775- or Estradiol+AZD1775-treated mice 3 weeks after induction of endometriosis. Quantitative analysis of percentage of Aniline Blue stained tissue after Masson’s trichrome staining. Data are presented as mean ± SD. Scale bar: 50 μm. * p < 0.05; ** p < 0.01; *** p < 0.001 compared to control tissue