Fig. 5

The effects of miR-182 on cell proliferation, cell cycle and cell apoptosis were abrogated by SGK3 overexpression in PCOS GCs and KGN cells. (A and B) The mRNA and protein levels of SGK3 in GCs from PCOS patients and normal controls were determined by qRT-PCR and western blot assay, respectively. (C and D) The mRNA and protein levels of SGK3 in IOSE80 and KGN cells were determined by qRT-PCR and western blot assay, respectively. (E) The protein level of SGK3 in PCOS GCs and KGN cells transfected with vector or SGK3 was measured through western blot assay. (F-O) PCOS GCs and KGN cells were divided into 4 groups: miR-con, miR-182, miR-182 + pcDNA and miR-182 + SGK3. (F and G) The mRNA and protein levels of SGK3 in transfected PCOS GCs and KGN cells were measured by qRT-PCR assay and western blot assay, respectively. (H and I) The proliferation of PCOS GCs and KGN cells was assessed by EdU assay and MTT assay. (J-M) Cell cycle and cell apoptosis in PCOS GCs and KGN cells were analyzed by flow cytometry analysis. (N and O) The protein level of BAX in PCOS GCs and KGN cells was examined with western blot assay.*P < 0.05