Fig. 4

Effect of NT-4 supplementation on transcriptome profiling of GV oocytes. a Number of different-expressed genes (DEGs) of each two groups among IVV, IVG, and IVG-NT group. Each group contains three samples from three independent experiments. b Venn diagrams showing overlap among DEGs of IVV, IVG, and IVG-NT group, upregulated and downregulated genes, respectively. c Four profiles of defined ‘improved genes’ according to the comparison of gene expression level among IVV, IVG, and IVG-NT groups. d GO enrichment analysis of ‘improved genes’. e Cytoscape plots from ‘improved genes’ and predicted transcription factors (with red circles) were given as an input to be used as potential regulators of the network. f KEGG enrichment analysis of ‘improved genes’ which simultaneously targeted by the predicted transcription factors. g GO enrichment analysis of ‘improved genes’ which simultaneously targeted by the predicted transcription factors. h, i, j Quantitative RT-PCR detection of Trp53 (h), Rac1 (i), Arf6 (j) transcripts in oocytes from Day 10 follicles. Each group contains three samples from three independent experiments. *, P < 0·05. **, P < 0·01. ***, P < 0·001