Fig. 6

Apoptosis of granulosa cells (GCs) in ovarian tissues were measured by TUNEL assay. a, d, g the nucleus was stained with DAPI, shown as blue fluorescence (40 ×). b, e, h Apoptotic cells shown as red fluorescence with TUNEL assay (40 ×). c, f, i Figures were merged by Image J (40 ×)