Table 2 Antibodies used in this study
Antibody | Host | Cat. no | Supplier | Dilution | Application |
|---|---|---|---|---|---|
β-tubulin | Rabbit | ab6046 | Abcam | 1:2000 | WB |
RIPK1 | Mouse | 610,459 | BD biosciences | 1:500 | WB/IF |
pRIPK1 | Rabbit | 83,613 | Cell signaling | 1:500 | WB |
pRIPK1 | Rabbit | 31,122 | Cell Signaling | 1:1000 | IF |
RIPK3 | Rabbit | NBP1–77299 | Novus | 1:500 | WB/IF |
pRIPK3 | Rabbit | 91,702 | Cell signaling | 1:500 | WB |
pRIPK3 | Rabbit | 57,220 | Cell Signaling | 1:1000 | IF |
MLKL | Rat | MABC604 | Merk | 1:500 | WB/IF |
pMLKL | Rabbit | ab196436 | Abcam | 1:500 | WB/IF |
E-cadherin | Rabbit | 3195 | Cell signaling | 1:200 | IF |
EEA1 | Rabbit | 2411 | Cell signaling | 1:250 | IF |
Lamp1 | Rat | NB100–77683 | Novus | 1:125 | IF |
Desmin | Goat | Sc7559 | Santa Cruz | 1:250 | IF |
Anti-rabbit IgG-HRP | Goat | SA002–500 | GenDEPOT | 1:10000 | WB |
Anti-mouse IgG-HRP | Goat | SA001–500 | GenDEPOT | 1:10000 | WB |
Anti-rat IgG-HRP | Goat | 62–9520 | Invitrogen | 1:10000 | WB |
Anti-rabbit IgG-Alexa Fluor 488 | Chick | A21441 | Invitrogen | 1:250 | IF |
Anti-rat IgG-Alexa Fluor 488 | Donkey | A21208 | Invitrogen | 1:250 | IF |
Anti-goat IgG-Alexa Fluor 488 | Rabbit | A21222 | Invitrogen | 1:250 | IF |
Anti-mouse IgG-Alexa Fluor 488 | Donkey | A31571 | Invitrogen | 1:250 | IF |