Fig. 2

Effect of a PI3K inhibitor on sPIF-enhanced ESC decidualization. Human ESCs were cultured in DMEM/F12 medium supplemented with E2, P4, and the PI3K inhibitor wortmannin (10 μM) in the presence or in the absence of sPIF (50 nM) for 15 days. A-B Total RNA was extracted after 15 days (D15) of cell decidualization. mRNA expression levels of IGFBP-1 (A), and connexin-43 (B) were quantified by RT-qPCR, as described in the Materials and Methods section. The data are presented as the mean ± SEM of 4 to 11 separate experiments. *: P < 0.05; **: P < 0.005 (Wilcoxon test). (a) sPIF vs. control (lacking sPIF); (b) wortmannin vs. control (lacking sPIF); (c) sPIF vs. sPIF + wortmannin