Table 1 qPCR primers
Gene Reference Name | Gene Full Name | Product Size (bp) | GenBank Accession # | Primer Sequence – Forward and reverse (5′-3′) | Efficiency (%) | References |
|---|---|---|---|---|---|---|
AMH | Anti-Müllerian Hormone | 270 | NM_173890.1 | F: CAGGGAAGAAGTCTTCAGCA R: AAGGTGGTCAAGTCACTCAG | 104.4 | [24] |
AMHRII | Anti-Müllerian Hormone Receptor Type II | 163 | NM_001205328.1 | F: GTGCTTCTCCCAGGTCATAC R: AATGTGGTCATGCTGTAGGC | 101.5 | [24] |
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | 153 | NM_001034034.2 | F: TTCCTGGTACGACAATGAATTTG R: GGAGATGGGGCAGGACTC | 99.5 | [25] |
PPIA | Peptidylpropyl isomerase A | 111 | NM_178320.2 | F: TCTTGTCCATGGCAAATGCTG R: TTTCACCTTGCCAAAGTACCAC | 103.7 | [25] |
DDX3Y | DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked | 225 | NM_001172595.1 | F: GGACGTGTAGGAAACCTTGG R: GCCAGAACTGCTACTTTGTCG | 101.5 | [26] |
USP9Y | Ubiquitin-specific peptidase 9, Y-linked | 285 | NM_001145509.1 | F: GCCAGATGACCAAGAAGCCCCA R: GGACTGTAAGGCCTAATAGCCTGGT | 101.1 | [26] |