Fig. 1

Quercetin reduces Cy-induced PMF loss. a-b Effects of PBS alone, quercetin alone, Cy alone, or Cy with quercetin on different stages of follicle development (n = 5 mice/group). Data are expressed as the means ± SEM. One-way ANOVA followed by Tukey’s tests was used for comparisons (***P < 0.001 compared with the Cy group; #P < 0.05, ##P < 0.01, ###P < 0.001 compared with the control group). c Representative images of haematoxylin and eosin staining ofthe ovarian histological sections from all experimental groups. Scale bars represent 100 μm (upper panel in each group) and 20 μm (lower panel in each group). Inset boxes denote areas shown in higher magnification to the below. Black arrows indicate the primordial follicles. d Representative images of DDX4-immunostained ovarian histological sections from all experimental groups. Inset boxes denote areas shown in higher magnification to the below. Red arrows indicate primordial follicles. Scale bars represent 500 μm