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Fig. 1 | Reproductive Biology and Endocrinology

Fig. 1

From: Anticentromere antibody induced by immunization with centromere protein a and Freund’s complete adjuvant may interfere with mouse oocyte meiosis

Fig. 1

Determination of anti-CENP-A antibody in mouse serum after treatment. a IIFT test for ANA-IgG to determine anti-CENP-A in mouse serum. Positive ANA was detected in serum samples from mice in the HA + CFA group, while none of the serum samples from the CFA and saline groups showed a fluorescence signal (n = 10 for each group, with an original magnification × 200). b Serum levels of anti-CENP-A antibody measured by the ELISA. Mice treated with CENP A and CFA exhibited significantly higher levels of anti-CENP-A antibody compared to mice treated with saline or CFA alone. Each histogram represents the mean ± SD obtained from 10 mice in each group, **p < 0.01 vs. CFA-treated mice, ***p < 0.001 vs. saline-treated mice

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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