Fig. 3
From: Hsp90 modulates human sperm capacitation via the Erk1/2 and p38 MAPK signaling pathways
Interaction between Hsp90 and Erk1/2 during human sperm capacitation. a. Hsp90 was detected in sperm lysates using western blotting and Co-IP with non-immune mouse IgG or antibodies against Erk1/2. No interaction was observed between non-immune mouse IgG and Hsp90, whereas a clear interaction was evident between Hsp90 and Erk1/2. 17-AAG significantly disrupted the interaction between Hsp90 and Erk1/2. b. The relative association of Hsp90 and Erk1/2 with or without 5 µM 17-AAG during human sperm capacitation