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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Hsp90 modulates human sperm capacitation via the Erk1/2 and p38 MAPK signaling pathways

Fig. 3

Interaction between Hsp90 and Erk1/2 during human sperm capacitation. a. Hsp90 was detected in sperm lysates using western blotting and Co-IP with non-immune mouse IgG or antibodies against Erk1/2. No interaction was observed between non-immune mouse IgG and Hsp90, whereas a clear interaction was evident between Hsp90 and Erk1/2. 17-AAG significantly disrupted the interaction between Hsp90 and Erk1/2. b. The relative association of Hsp90 and Erk1/2 with or without 5 ┬ÁM 17-AAG during human sperm capacitation

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