Fig. 7

LPS activates the MEG3/miR-93-5p/PTEN signalling pathway in Leydig cells. (A) qPCR and western blot analysis of PTEN expression in Leydig cells divided into four groups: mimics NC, LPS + mimics NC, LPS+ miR-93-5p mimics and LPS + miR-93-5p mimics+plvx-MEG3. (B) qPCR and western blot analysis of PTEN expression in Leydig cells transfected with shPTENs and shNC for 48 h. (C) ELISA analysis of secretion of TNFα and IL6 in Leydig cells. (D) ELISA analysis of testosterone production in Leydig cells. (E) CCK8 analysis of cell viability in Leydig cells. (F) EdU analysis of cell proliferation in Leydig cells. For C-F, Leydig cells were divided into five groups: mimics NC, LPS + mimics NC, LPS+ miR-93-5p mimics, LPS + miR-93-5p mimics+plvx-MEG3 and LPS + miR-93-5p mimics+plvx-MEG3 + shPTEN. β-Actin was measured as an internal control for qPCR analysis. “**” represents p < 0.01, which indicates statistically significant differences between two groups in Student’s t-test