Fig. 3

MEG3 absorbed miR-93-5p and miR-93-5p alleviated the role of LPS in Leydig cells. (A and B) Luciferase reporter system analysis of the interaction between MEG3 and miR-93-5p. (C) qPCR analysis of miR-93-5p expression in Leydig cells treated with different concentrations of LPS (0, 50, 100, 200, 400 μg/ml) for 48 h. (D) qPCR analysis of miR-93-5p expression in Leydig cells transfected with miR-93-5p mimics, mimics NC, miR-93-5p inhibitor and inhibitor NC for 48 h. (E) ELISA analysis of secretion of TNFα and IL6 in Leydig cells. (F) ELISA analysis of testosterone production in Leydig cells. (G) CCK8 analysis of cell viability in Leydig cells. (H) EdU analysis of cell proliferation in Leydig cells. For E-H, Leydig cells were divided into four groups: mimics NC, miR-93-5p mimics, mimics NC + LPS and miR-93-5p mimics+LPS. U6 was measured as an internal control for qPCR analysis. “**” represents p < 0.01, which indicates statistically significant differences between two groups in Student’s t-test