Table 2 Primers used for qRT-PCR
Gene | Forward primers(5′-3′) | Reverse primers(5′-3′) |
|---|---|---|
Kiss1 (Rat) | AGCTGCTGCTTCTCCTCTGT | AGGCTTGCTCTCTGCATACC |
TTF1 (Rat) | GGACGTGAGCAAGAACATGG | GCCGACAGGTACTTCTGCTG |
GnRH (Rat) | CCGCTGTTGTTCTGTTGACTGTG | GGGGTTCTGCCATTTGATCCTC |
β-actin (Rat) | TGCCGCATCCTCTTCCT | GGTCTTTACGGATGTCAACG |
TTF1 (mouse) | GGGCCAGGTCTCTAGCCTATC | CTCACCAGGTCCGACCATAAA |
Kiss1 (mouse) | CTCTGTGTCGCCACCTATGG | AGGCTTGCTCTCTGCATACC |
GnRH (mouse) | TGATCCTCAAACTGATGGCCG | CGCAACCCATAGGACCAGTG |
β-actin (mouse) | AAGATCAAGATCATTGCTCCTCC | GACTCATCGTACTCCTGCTTGC |