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Fig. 5 | Reproductive Biology and Endocrinology

Fig. 5

From: Downregulation of TTF1 in the rat hypothalamic ARC or AVPV nucleus inhibits Kiss1 and GnRH expression, leading to puberty delay

Fig. 5

Knockdown of the TTF1 gene inhibited the expression of Kiss1 and GnRH in both the ARC and AVPV. Twenty-one-day-old female rats were infected with LV-TTF1-shRNA or LV-NC (n = 6). a-c mRNA levels of TTF1, Kiss1 and GnRH in the ARC were detected with qRT-PCR at PND25, PND35 and PND42. d-f Expression of the TTF1, Kiss1 and GnRH genes in the AVPV was detected with qRT-PCR (n = 6) at PND25, PND35 and PND42. β-actin was used as the internal reference. g-i Expression of the TTF1 and Kiss1 proteins in the ARC and AVPV was detected by western blotting at PND25, PND35 and PND42. KD for the TTF1-knockdown group, NC for the lentivirus negative control group. ARC, Arcuate nucleus. AVPV, Anterior ventral periventricular nucleus. The results are shown as the mean ± S.E.M. *P < 0.05, **P < 0.01, ***P < 0.001

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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