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Table 4 Immunohistochemical analysis

From: Pretreatment with melatonin improves ovarian tissue cryopreservation for transplantation

 

Control

Melatonin

p-value

Cleaved caspase 3 F

11.30 ± 1.87

13.70 ± 1.79

0.38

Cleaved caspase 3 CL

5.88 ± 0.82

24.50 ± 2.06

< 0.001*

TUNEL F

0.40 ± 0.14

0.04 ± 0.02

< 0.001*

TUNEL CL

1.58 ± 0.23

0.10 ± 0.03

< 0.001*

Ki67 F

1.46 ± 0.29

4.09 ± 0.55

0.002*

Ki67 CL

1.67 ± 0.33

5.27 ± 0.54

< 0.001*

Estrogen receptor F

2.41 ± 0.93

5.37 ± 0.73

0.02*

Estrogen receptor CL

2.25 ± 0.38

6.43 ± 0.85

< 0.001*

Progesterone receptor F

4.26 ± 0.53

4.65 ± 1.13

0.77

Progesterone receptor CL

16.62 ± 1.07

13.47 ± 1.72

0.14

von Willebrand factor

1.69 ± 0.28

3.19 ± 0.38

0.003*

  1. Immunohistochemical analysis comparing the use of melatonin added to the cryopreservation medium or not, in rat ovarian slide, autologous cryopreserved grafts 30 days after transplantation (n = 10 in each group); * p < 0.05; F Follicle, CL Corpora lutea