Table 1 Primers used for RT-PCR validation
Gene symbol | Primer sequence (5’-3’) |
|---|---|
miRNA-4497 | F:GCGCGCTCCGGGACGG R:AGTGCAGGGTCCGAGTATT RT:GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGCCCAG |
U6 | F:AGAGAAGATTAGCATGGCCCCTG R:AGTGCAGGGTCCGAGGTATT RT:GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAATA |
GAPDH | F:AATCCCATCACCATCTTCCA R:TGGACTCCACGACGTACTCA |
AGBL4 | F:CGCTTCCGAGTCTGGTTCAA R:CCATAGGGGCCATCCCATCT |
ESPN | F: CAGAGTGCAGGACAAAGACAA R: GCAGCGTAGTGGATAGGCAG |
HTT | F: AAACTTCTGGGCATCGCTATG R: GTTGAGGCATTCGTCAGCCA |
ISOC2 | F: ATCCTCTGTCCTGTTCCTGTG R: CTGAGACGATCTGTGGGAAGTA |
NRXN2 | F: CAGCACGAGGATGGATCGC R: GCCCACGTTAAAGATCACCCC |
RCC2 | F: AAGGAGCGCGTCAAACTTGAA R: GCTTGCTGTTTAGGCACTTCTT |
TAF6L | F: CGGCGGTTTGTGGAGATCC R: CCTCTCTCAGACGATAGCACAC |
WASF2 | F: TAGTAACGAGGAACATCGAGCC R: AAGGGAGCTTACCCGAGAGG |
C20orf160 | F: ACCACGGCGGAGCAGGAC R: TAGGAGGCGGCGGCGATC |
SP1 | F: GGAAGGAGAAAACAGCCCAGAT R: GAGCCCCTTCCTTCACTGTCT |
BMP8A | F:GTGTCAGGACAAGTCCCCTT R:TCCTGCAATGGCAGATAGGC |
CXCR5 | F:CACGTTGCACCTTCTCCCAA R:GGAATCCCGCCATGGTAG |