Fig. 3

SP1 levels are inversely correlated with miRNA-4497 levels in the placental villus tissues. a and b Decreases of the SP1, CXCR5 and BMP8A protein levels in RM (n = 8) compared with those in normal chorionic villus tissues (n = 8). c The protein levels of SP1 and CXCR5, but not BMP8A, are inversely correlated with the miRNA-4497 levels in chorionic villus tissues from RM and normal control patients. d The results of immunohistochemical analysis of SP1 expression in the villus tissues of RM and normal controls. A significantly low SP1 level was detected in the villus tissues of RM. There were no significant change in the expression of CXCR5 and BMP8A. Villus tissues were counterstained with haematoxylin. SP1, CXCR5 and BMP8A proteins were stained in brown colour. *, P<0.01, ***, P<0.001