Fig. 6
Inhibitor effects on on long-lasting [Ca2+]i oscillations. a Cytoplasmic [Ca2+]i dynamic changes after inhibitor addition. b Development of inhibitor addition following [Ca2+]i oscillations initiation. PA indicates survival of embryos of all MII oocytes. PN indicates pronuclear embryos of surviving oocytes. 2-Cell indicates cleaved embryos of pronuclear embryos. RR: 10 μM Ruthenium Red; Tha: 1 μM Thapsigargin; GSK: 1 mM GSK-7975A; Mib: Mibefradil; N: 1 μM NS-8593. The significance of differences between groups was analyzed by the Chi-square test and p < 0.05 (*) was considered statistically significant. (X) indicates data unavailable