Fig. 2
Effect of SERCAs inhibitor Thapsigargin on oocyte activation. a Cytoplasmic ([Ca2+]i) dynamic changes of Thapsigargin-inhibited oocyte during activation. b Mitochondrial membrane potential of 1 μM Thapsigargin-inhibited oocytes during activation. c Mitochondrial membrane potential fluorescence intensity of 1 μM Thapsigargin-inhibited oocytes. The green and red curves represent labeling with JC-1, indicating relative fluorescence intensities of low membrane potential and high membrane potential, respectively. The black curve shows the ratio of high membrane potential to low membrane potential indicating relative mitochondrial membrane potential. d Living cell observation of 1 μM Thapsigargin inhibition of oocyte spindle formation e Living cell observation of 1 μM Thapsigargin inhibition of oocyte subcortical F-actin distribution