Table 1 Primers sequences designed for Real-time PCR
Genes | Sequence (5′➔3′) | Annealing temperature (°C) | Product length | |
|---|---|---|---|---|
Rn-ANGPT2 (Rattus norvegicus angiopoietin 2), NCBI accession number NM_134454.1 | Forward | GCAGCGTTGACTTCCAGAGA | 60 | 199 |
Reverse | ATACAGAGAGTGTGCCTCGC | |||
Rn-KDR (Rattus norvegicus kinase insert domain receptor) NCBI accession number NM_013062.1 | Forward | AGATGCGGGAAACTACACGG | 60 | 184 |
Reverse | GGGAGGGTTGGCATAGACTG | |||
Rn-β-actin (Rattus norvegicus Beta Actin) NCBI accession number NM_031144.3 | Forward | TGACAGGATGCAGAAGGAGA | 60 | 104 |
Reverse | TAGAGCCACCAATCCACACA | |||