Fig. 3

Examination of the effect of CDH6 knockdown on Ishikawa cell adhesive capacity. Ishikawa cells were transfected with either scrambled control (50 nM) or CDH6 siRNA (10, 20 or 50 nM) before HTR8/SVneo spheroid adhesion assay. aCDH6 knockdown was determined by qPCR. Expression levels were normalized to 18S (n = 7). b Immunoblotting was also used to determine the CDH6 expression. Blots were co-probed with an anti-GAPDH antibody to confirm equivalent protein loading of each sample. c A siRNA concentration-dependent reduction of the adhesion was observed in Ishikawa cells with the highest concentration of CDH6 siRNA (50 nM) significantly compromised the spheroid adhesion compared to scrambled control. Data were presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001